Quantum-Si, has released its V4 Sequencing Kit, which is designed to improve proteomic coverage and enable new applications in protein analysis. The kit is compatible with the company’s Platinum® and Platinum® Pro benchtop instruments.
According to the company, the V4 kit introduces several advancements over its predecessor, the V3 kit. Among these are recognition of glycine, improved detection of alanine and serine, an enzyme capable of cleaving amino acids positioned before proline, and updated algorithms for higher precision and mixture handling.
The technical updates collectively expand the range of proteins and peptides that can be sequenced.
Quantum-Si reports that the kit enables:
- Sequence Beyond Proline: V4 Sequencing Kit enables the analysis of proline-rich domains common in antibodies and membrane proteins.
- Expanded Amino Acid Coverage: New recognizers, enzyme and improved bioinformatics offers an 85% increase in sequenceable peptides compared to the V3 Sequencing Kit.
- Sample Mixture Precision: Expanded coverage and improved bioinformatics enables barcoding of up to 24-plex and improved analysis of protein mixtures common in immunoprecipitation workflows.
The performance of the V4 Sequencing Kit is a significant leap forward over the prior version of our chemistry and will open up new applications such as antibody sequencing and expand the range of complex mixtures customers can analyze. In addition, as part of this version 4 sequencing kit release, we are enabling our barcoding customers to capture further cost savings with an expanded set of 24 barcodes without sacrificing on analytical performance.
Jeff Hawkins, President and CEO of Quantum-Si,
The launch underscores a growing emphasis on proteomics technologies that aim to provide more detailed insights into protein structure and function. While genomics has long benefited from rapid sequencing innovations, single-molecule protein sequencing remains an emerging field with potential implications for antibody engineering, drug discovery, and clinical diagnostics.