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The setup of qPCR reactions is a vital yet time-consuming step in the process of next-generation sequencing (NGS) library quantification. With the development of irefly protocols, researchers can now automate the setup of qPCR reactions using KAPA Library Quantification Kits, offering significant time savings and improved consistency.
These protocols enable qPCR reactions to be easily prepared for 1-12 columns of samples in a 96-well plate. KAPA Library Quantification Kits, optimized for Illumina sequencing platforms, contain all necessary reagents, including the KAPA SYBR FAST qPCR Master Mix, DNA standards, and platform-specific primers. This ensures the reliable and reproducible quantification of NGS libraries.
By leveraging SPT Labtech’s firefly platform, which combines air-displacement pipetting with non-contact dispensing, the entire process becomes more streamlined. Its intuitive software brings the power of automation to labs of all sizes, accelerating genomic research and enabling researchers to focus on discovery rather than tedious manual tasks.